Microscope Skill Worksheet

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Analisis Soalan Paper 3 Past years

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Paper 3 Qs2~Enzyme

The question is about one of the factors which affect enzyme activity which is TEMPERATURE.

And when we talk about enzymes which can be used in Bio Lab, it is usually refer to AMYLASE which can be prepared by using SALIVA.

Please take note the bullets required in your planning...

Please take note about the variables..

Responding variable: the time taken for starch to hydrolyse (also accepted)

Controlled Variable: ONLY ONE.

Apparatus & Materials: All in one..

**Can use water bath as apparatus.

***Need to produce ONE sentence for Safety measures..

Experiments Setup

You may use this apparatus setup for experiments involving YEAST..A manometer..This apparatus for macronutrients experiment..
This one is for energy value..May include 'wind shield' in a diagram!
Photosynthesis experiment ~ whatever manipulated variables are...

Repost~ Define Operationally

Define Operationally

It must be done BASED ON EXPERIMENT GIVEN....

GempurBio3- Qs 2 Template

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May use this template to answer Question 2.

GempurBio 3-An analysis

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Sample Q&A Paper 3 Qs2

Its Transpiration....
You may produce a Diagram of a set up of apparatus...but...make sure it has at least 5 labels to entitle point (not marks) for your procedure !

It is POTOMETER..

// means either or...

The underlined ones are A MUST..without those, your marks will be reduced by 1.

Observe how the procedures are written above..

Identify which are K1, K2, K3, K4 and K5

Make sure your TABLE must have MV, RV and units.

NO CONCLUSION is required.

Paper 3 Technique Latest

Starting 2010, The format of answering for Paper3 QUESTION 2 has changed slightly. It is not announced widely which I dont think it is wise..Below is the marks allocated for your answers. You have to observe and follow closely the requirement of the question...meaning that ..you have to read the question CAREFULLY...

Make sure you answer according to the bullets provided..

PAPER 3 , QUESTION 2, SPM 2010

PAPER 3, QUESTION 2, SPM 2009

Energy Value

To determine the energy value in food samples:

i. ground nut

ii. cashew nut
Problem Statement: What are the energy values of different food samples?

Hypothesis: Cashew nut has a higher energy value than ground nut.//vice versa

Variables:

Manipulated: Types of nuts/type of food samples

Responding: Energy value

Fixed: mass of water

Apparatus & Material:

Ground nut, cashew nut, pin, plasticine, cotton wool, thermometer, boiling tube, bunsen burner, electronic balance, distilled water, retort stand, wind shield

Procedure:

List down the procedure. Make sure you include sentences of:

1. How to set up the apparatus. **At least 3 sentences.

2. How to manipulate variables

3. How to get a responding variable

4. How to get fixed variable.

5, Safety measures such as:

-use wind shield to avoid disturbances of wind which will affect the exp result.

**List only one should be sufficient.

This is called ' A simple calorimeter'

Setting up of apparatus

Result:

Produce a Table with manipulated variable and responding variable in a row and column respectively.

Do not forget the unit!!!

Do not forget the formula to calculate the energy value (J/g) of food.

*State the formula please ...

Paper 3 Question 2

Kuala Lumpur Trial 2010 Paper 3 Question 2- To consider!

The total surface area to volume ratio and the diffusion rate over large distances are limiting factors to cellular activities in multicellular organisms.

Based on the above information, design a laboratory experiment to study the relationship between the total surface area to volume ratio and how it affects the rate of diffusion. Use the suitable plant tissue to design your experiment.

The planning of your experiment must include the following aspects :

Problem statement

Aim of investigation

Hypothesis

Variables

List of apparatus and materials

Technique used

Experimental procedure or method

Presentation of data

Conclusion [17 marks]

Suggested Answer:

Aim:To study the relationship between the total surface area to volume ratio and its effect on the rate of diffusion.

Problem Statement: How does the total surface area to volume ratio affect the the rate of diffusion ?

Hypothesis:The bigger the size of an object, the lower the total surface area to volume ratio and the lower the rate of diffusion.

Variables:

Manipulated variable – size of potato cube

Responding variable – percentage of stained area

Fixed variable- coloured solution, time

Apparatus and Materials:

Potatoes, coloured solution, Beakers, white tile, ruler, knife

TECHNIQUE : Observe, estimate and record the area of potato being stained using a graph paper .

Procedure:

Sample answer:

1. Use a knife to cut a potato cube of sides 1cm, 2cm and 3cm.

2. Calculate the total surface area and volume of the cube

3. Immerse all the cubes in a beaker containing coloured solution

for 30 minutes. Ensure all cubes are immersed in the coloured solution at the same time

4. Remove all the cubes after 30 minutes.

5. Cut each cube into two halves.

6. Observe the area stained for each cube.

7. Estimate the percentage of area stained using a graph paper and record the results in a table.

Conclusion : Rewrite hypothesis sentence

Paper 3 Question 2

SBP Trial 2010 Paper 3 Question 2 -to consider:

Transpiration is the loss of water vapour from plants, especially in leaves. Transpiration occurs mostly through the stomata. The amount of water lost by a plant depends on its size,surrounding light intensity, temperature, humidity and wind speed.

Diagram 3 shows the movement of water in a terrestrial plant.

Based on the information, design an experiment to be conducted in the laboratory to investigate the effect of the number of leaves on the rate of transpiration in a hibiscus plant.

The planning of your experiment must include the following aspects:

_ Objective of investigation

_ Hypothesis

_ Variables

_ List of materials and apparatus

_ Technique used

_ Experimental procedures

_ Presentation of data

_ Conclusion [17 marks]

TIPS:

***IDENTIFY THE VARIABLES

Aim : To investigate the effect of the number of leaves on the rate of transpiration in a hibiscus plant.

Problem Statement: HOW does the number of leaves affect the rate of transpiration (in hibiscus plants)?

Hypothesis:

The more the number of leaves the higher rate of transpiration. OR

When the number of leaves increases the rate of transpiration increases.

VARIABLES:

Manipulated variable: Number of leaves / stomata

Responding variable: Distance travelled by air bubble (in five minutes)// The rate of transpiration

Controlled variable: Type of (terrestrial) plant / hibiscus // Light intensity //Surrounding temperature

Materials & Apparatus:

Materials: *Hibiscus shoot / plant, water, and plasticine.

Apparatus: *Ruler / weighing balance, capillary tube + rubber tubing // potometer //

stoppered conical flask, beaker / basin, (sharp) knife, stopwatch, string /

marker pen and tissue paper / filter paper.

Technique:

Recording the distance travelled by air bubble in five minutes using a stopwatch.

OR

Calculating and record the rate of transpiration by the formula:

Rate of transpiration = Distance travelled by air bubble/Time

Procedure:

Able to state five procedures P1, P2, P3, P4 and P5 correctly.

P1 : How to Set Up The Apparatus (5P1)

P2 : How to Make Constant The Control Variable (1P2)

P3 : How to Manipulate The Manipulated Variable (1P3)

P4: How to Record The Responding Variable (2P4)

P5 : Precaution / Accuracy (2P5)

PROCEDURE:

1. (Diagram of experimental setup with at least 5 functional labels). P1

2. Obtain a hibiscus shoot and immediately immerse in water. P1/ P5

3. By using a sharp knife, cut off 4 cm of the hibiscus stem under water. P5

4. Fill in the capillary tube with attached rubber tubing / potometer with water. P1

5. Fix in the stem of the hibiscus shoot into the rubber tubing / potometer.Make sure no air bubble trapped. P1/ P5

6. Immerse the capillary tube / potometer in a beaker of water. P1

7. Wipe dry the leaves with tissue papers. P5

8. Leave the setup for 5 minutes (for the plant to adapt with the new environment). P5

9. Lift the capillary tube from the water to trap a column of air bubble // Trap an air

bubble in the capillary tube / potometer. P1

10. Tie a string on the capillary tube to mark the initial position of the air bubble. P1

11. Start the stopwatch. P1

12. After 5 minutes tie another string to mark the final position of the air bubble. P1

13. Repeat step 12 to get another reading. P5

14. Measure both distances by using a ruler. P4

15.Calculate the average distance traveled by the air bubble in 5 minute. P4

16.Record in a table // Tabulate the data. P4

17. By using the same plant,repeat steps 7 until 13 by removing one leave each time.P2/ P3

18. Calculate the rate of transpiration. P4

Result:

Conclusion : Rewrite hypothesis sentence.

Analysis of Paper Three 2005-2009

Please use your own judgement to decide what are the possibilities of Paper 3 questions this year 2010...

*You may use this simple analysis to decide !...If you dare to forecast :-)

2005:

Qs 1- Energy value (F4)

Qs 2-Isotonic solution (F4)

2006:

Q1-Different CONCENTRATION OF SUBSTRATE affects on the rate of enzyme PEPSIN (F4)

Q2- AGAIN---Isotonic solution (F4)

2007:

Q1- Effect of LIGHT INTENSITY on the rate of photosynthesis.(F4)

Q2-Effect of TEMPERATURE on THE RATE OF ANAEROBIC RESPIRATION IN YEAST. (F4)

2008:

Q1- The population of Pleurococcus affected by abiotic factor :LIGHT INTENSITY. (F4)

Q2- Molecules size and its affect on rate of diffusion through a semi permeable membrane. (F4)

2009:

Q1- AGAIN---REPEAT 2007 !! Effect of light intensity on the rate of photosynthesis. (F4)

Q2- Effect of cold water and hot water on enzyme activity. (F4)

So...you see guys...Its not easy to forecast this paper..Looks like it is always from the Form 4 Chapters!... enzymes, photosynthesis, isotonic...have been repeatedly asked!

I would advise you to know the technique of answering this paper WELL..Then you dont have to worry what questions will be asked because you already master the format of answering it..

Paper 3 is a must score paper...Its not difficult..The questions are sure within the scope of Biology syllabus.

Maybe what you have done for your PEKA Biology could help you to understand more the experiments involved..

Or else you can start imagining doing experiment, but the format must always be like this:

Aim:

Problem Statement:

Hypothesis:

Variables:

Apparatus &Matreials:

Technique:

Procedure:

Result:

Conclusion:

HAPPY REVISING :-)

Paper 3 Question 2

Here are some possibilities of Question 2 PAPER3.

FROM CHAPTER 9: ENDANGERED ECOSYSTEM

1. Diagram 1 shows an area where different activities are being carried out. The river flowing at the area receives the wastes from the town and also from the factories. In conjunction with their studies of the effects of human activities on the environment, a group of students carried out an experiment to study the level of water pollution in three stations A, B and C, along the river.

Plan an investigation which you could carry out in the laboratory to determine the level of pollution in stations A, B and C.The planning of your experiment must include the following aspects:

● Problem statement

● Aim of investigation

● Hypothesis

● Variables

● List of apparatus and materials

● Technique

● Procedure

● Presentation of data

● Conclusion

FROM CHAPTER 8: DYNAMIC ECOSYSTEM

2.Interaction between the two components, the biotic and abiotic in an ecosystem produces a stable ecosystem.Based on the above information, design an experiment to investigate how the temperature does affects the growth of yeast.

The planning of your experiment must include the following aspects:

● Problem statement

● Aim of investigation

● Hypothesis

● Variables

● List of apparatus and materials

● Technique

● Procedure

● Presentation of data

● Conclusion

FROM CHAPTER 6; NUTRITION

3. The rate of photosynthesis is influenced by different environment factors. Based on the above situation; plan a laboratory experiment to determine the effect of temperature on the rate of photosynthesis.

The planning of your experiment must include the following aspects:

· Statement of identified problem

· Objective of study

· Variables

· Statement of hypothesis

· List of materials and apparatus

· Technique used

· Experimental procedure

· Presentation of data

· Conclusion

FROM CHAPTER 8: DYNAMIC ECOSYSTEM

4. The population of crow is increasing in Nilai Village due to the existence of dumping site. The villager complained the birds’ dropping caused corrosion to their vehicle paint.

A group of biologists decides to carry out a study to find out the population size of the crow before deciding the appropriate approach to overcome the problem faced by the community. The population size of crow in Nilai Village is compared to the population of crow in Kluang Village which is 10km away.

The planning of your experiment must include the following aspects:

● Problem statement

● Aim of investigation

● Hypothesis

● Variables

● List of apparatus and materials

● Technique

● Procedure

● Presentation of data

● Conclusion

FROM CHAPTER 7 : RESPIRATION

5. During vigorous exercise such as running, the muscles require more oxygen and glucose to release energy during cellular respiration. Therefore the rate of respiration and heartbeat change. Design an experiment to study the effect of vigorous exercise on the respiration and heartbeat rates.

The planning of your experiment must include the following aspects

• Problem statement

● Aim of investigation

● Hypothesis

● Variables

● List of apparatus and materials

● Technique

● Procedure

● Presentation of data

● Conclusion

FROM CHAPTER 6: NUTRITION

6. Plant needs macronutrients and micronutrients to grow healthy and reproduce. Nitrogen as in nitrate, phosphorus and potassium are most important.By using a suitable plant that can absorb nutrient from nutrient solution (aquaculture), design an experiment to show the effect of nitrogen, phosphorus and potassium deficiency on this plant.

Your experimental planning need to include the following aspects:

· Statement of identified problem

· Objective of study

· Variables

· Statement of hypothesis

· List of materials and apparatus

· Technique used

· Experimental procedures

· Presentation of data

* Conclusion

FROM CHAPTER 3; CHEMICAL COMPOSITION

7. A group of students did a study on the function of enzymes as an organic catalyst that can regulate and increase the rate biochemical reactions in the cell. Enzymes are very sensitive to a change in temperature and functions efficiently at an optimum temperature. Design an experiment to study the effect of different substrate concentration on the activity of salivary amylase on starch.

The planning of your experiment must include the following aspects:

· Statement of identified problem

· Objective of study

· Variables

· Statement of variables

· List of materials and apparatus

· Technique used

· Experimental procedure

· Presentation of data

* Conclusion