Diagram 3 shows the movement of water in a terrestrial plant.
Based on the information, design an experiment to be conducted in the laboratory to investigate the effect of the number of leaves on the rate of transpiration in a hibiscus plant.
The planning of your experiment must include the following aspects:
_ Objective of investigation
_ Hypothesis
_ Variables
_ List of materials and apparatus
_ Technique used
_ Experimental procedures
_ Presentation of data
_ Conclusion [17 marks]
TIPS:
***IDENTIFY THE VARIABLES
Aim : To investigate the effect of the number of leaves on the rate of transpiration in a hibiscus plant.
Problem Statement: HOW does the number of leaves affect the rate of transpiration (in hibiscus plants)?
Hypothesis:
The more the number of leaves the higher rate of transpiration. OR
When the number of leaves increases the rate of transpiration increases.
VARIABLES:
Manipulated variable: Number of leaves / stomata
Responding variable: Distance travelled by air bubble (in five minutes)// The rate of transpiration
Controlled variable: Type of (terrestrial) plant / hibiscus // Light intensity //Surrounding temperature
Materials & Apparatus:
Materials: *Hibiscus shoot / plant, water, and plasticine.
Apparatus: *Ruler / weighing balance, capillary tube + rubber tubing // potometer //
stoppered conical flask, beaker / basin, (sharp) knife, stopwatch, string /
marker pen and tissue paper / filter paper.
Technique:
Recording the distance travelled by air bubble in five minutes using a stopwatch.
OR
Calculating and record the rate of transpiration by the formula:
Rate of transpiration = Distance travelled by air bubble/Time
Procedure:
Able to state five procedures P1, P2, P3, P4 and P5 correctly.
P1 : How to Set Up The Apparatus (5P1)
P2 : How to Make Constant The Control Variable (1P2)
P3 : How to Manipulate The Manipulated Variable (1P3)
P4: How to Record The Responding Variable (2P4)
P5 : Precaution / Accuracy (2P5)
PROCEDURE:
1. (Diagram of experimental setup with at least 5 functional labels). P1
2. Obtain a hibiscus shoot and immediately immerse in water. P1/ P5
3. By using a sharp knife, cut off 4 cm of the hibiscus stem under water. P5
4. Fill in the capillary tube with attached rubber tubing / potometer with water. P1
5. Fix in the stem of the hibiscus shoot into the rubber tubing / potometer.Make sure no air bubble trapped. P1/ P5
6. Immerse the capillary tube / potometer in a beaker of water. P1
7. Wipe dry the leaves with tissue papers. P5
8. Leave the setup for 5 minutes (for the plant to adapt with the new environment). P5
9. Lift the capillary tube from the water to trap a column of air bubble // Trap an air
bubble in the capillary tube / potometer. P1
10. Tie a string on the capillary tube to mark the initial position of the air bubble. P1
11. Start the stopwatch. P1
12. After 5 minutes tie another string to mark the final position of the air bubble. P1
13. Repeat step 12 to get another reading. P5
14. Measure both distances by using a ruler. P4
15.Calculate the average distance traveled by the air bubble in 5 minute. P4
16.Record in a table // Tabulate the data. P4
17. By using the same plant,repeat steps 7 until 13 by removing one leave each time.P2/ P3
18. Calculate the rate of transpiration. P4
Result:

Conclusion : Rewrite hypothesis sentence.
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